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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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Data corrente: |
29/09/2003 |
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Data da última atualização: |
02/04/2013 |
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Autoria: |
SILVA-WERNECK, J. O.; MONNERAT, R. G. |
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Título: |
Metodologias para caracterização de isolados de Bacillus thuringiensis. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2001. |
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Páginas: |
5 p. |
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Série: |
(Embrapa Recursos Genéticos e Biotecnologia. Circular Técnica, 10). |
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Idioma: |
Português |
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Palavras-Chave: |
Caracterização de isolados; Metodologia. |
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Thesagro: |
Bacillus Thuringiensis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00577nam a2200169 a 4500
001 1184824
005 2013-04-02
008 2001 bl uuuu u0uu1 u #d
100 1 $aSILVA-WERNECK, J. O.
245 $aMetodologias para caracterização de isolados de Bacillus thuringiensis.
260 $aBrasília: Embrapa Recursos Genéticos e Biotecnologia$c2001
300 $a5 p.
490 $a(Embrapa Recursos Genéticos e Biotecnologia. Circular Técnica, 10).
650 $aBacillus Thuringiensis
653 $aCaracterização de isolados
653 $aMetodologia
700 1 $aMONNERAT, R. G.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDAO, F. Z. |
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Afiliação: |
Helena F. R. de A. Saraiva, UFF; Ribrio I. T. P. Batista, UFF; Vivian A. P. Alfradique, UFF; Pedro H. N. Pinto, UFF; Lilian S. Ribeiro, UFF; Clara S. Oliveira, UFF; Joanna M. G. de Souza-Fabjan, UFF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Z. Brandão, UFF. |
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Título: |
L-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Theriogenology, v. 105, p. 150-157, jan. 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies. MenosAbstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C... Mostrar Tudo |
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Palavras-Chave: |
In vivo embryo production; Santa ines. |
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Thesagro: |
Ovis Aries. |
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Thesaurus NAL: |
cryopreservation; gene expression. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02970naa a2200289 a 4500
001 2085659
005 2023-01-27
008 2018 bl uuuu u00u1 u #d
100 1 $aSARAIVA, H. F. R. de A.
245 $aL-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression.$h[electronic resource]
260 $c2018
520 $aAbstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies.
650 $acryopreservation
650 $agene expression
650 $aOvis Aries
653 $aIn vivo embryo production
653 $aSanta ines
700 1 $aBATISTA, R. I. T. P.
700 1 $aALFRADIQUE, V. A. P.
700 1 $aPINTO, P. H. N.
700 1 $aRIBEIRO, L. S.
700 1 $aOLIVEIRA, C. S.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDAO, F. Z.
773 $tTheriogenology$gv. 105, p. 150-157, jan. 2018.
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